Isolation and antigenic characteristics of axial filaments from the Reiter Treponeme.
نویسندگان
چکیده
Axial filaments were isolated and purified from Reiter treponemes after detergent solubilization of the cells" outer envelope. The axial filaments were separated from the spirochetal cells by shearing, purified by density gradient centrifugation, and fragmented by ultrasonication. Acrylamide gel electrophoresis of dissociated filaments revealed two major protein bands. Gel diffusion precipitin tests and immunoelectrophoresis between a purified axial filament suspension and anti-Reiter treponeme serum gave a single precipitin line. Checkerboard complement fixation tests also gave results consistent with a single antigen-antibody system. Tests with immune sera to other cultivable spirochetes were positive with some and negative with others. In addition, strongly positive reactions were obtained in complement fixation and precipitin tests with sera from rabbits and humans with syphilis and other treponematoses. However, both serological tests gave reactions of partial identity between the antigen(s) of Reiter treponeme axial filaments and those of the pathogenic treponemes. It was concluded from these studies that the axial filaments were probably the cellular locus of the so-called "Reiter protein" antigen of syphilis serology.
منابع مشابه
Reiter protein antigen. I. Relationship of some physical and chemical characteristics to serological activity.
The Italian workers D'Alessandro, Oddo, Comes, and Dardanoni (1949) and D'Alessandro and Dardanoni (1953) described the isolation of four serologically active components of Reiter's treponeme. These contributions represent milestones in the sero-diagnosis of syphilis since they were the initial systematic investigations into the serological components of treponemes. One of the components was co...
متن کاملAn account of the so-called Reiter treponeme (history, isolation, cultivation, specificity, and utilization).
متن کامل
Reiter's protein complement-fixation test; report of a trial in 1,000 unselected cases.
In recent years there has been a tendency to experiment with antigens derived from treponemata in the diagnostic tests for treponemal disease in the hope that they might prove more sensitive and more specific than the cardiolipin antigen which is now the standard preparation. These antigens have been prepared either from a virulent strain of Treponema pallidum or from the avirulent Reiter strai...
متن کاملResults of parallel tests with the Reiter protein complement-fixation test, the treponemal immobilization test. and the treponemal Wassermann reaction on 1,046 sera.
The Treponemal Immobilization Test (TPI) is now generally accepted as a highly sensitive and specific test for treponemal infection, but its complexity and the difficulties inherent in the use of a living organism as antigen have restricted its performance to specialized laboratories. These limitations have led to the re-investigation of the cultivable strains of treponemes as antigens for the ...
متن کاملResults of Parallel Tests with the Reiter Protein
The Treponemal Immobilization Test (TPI) is now generally accepted as a highly sensitive and specific test for treponemal infection, but its complexity and the difficulties inherent in the use of a living organism as antigen have restricted its performance to specialized laboratories. These limitations have led to the re-investigation of the cultivable strains of treponemes as antigens for the ...
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عنوان ژورنال:
- Infection and immunity
دوره 11 2 شماره
صفحات -
تاریخ انتشار 1975